Development and evaluation of microsatellite markers for the gynodioecious shrub Daphne jezoensis (Thymelaeaceae)1
نویسندگان
چکیده
UNLABELLED PREMISE OF THE STUDY Ten microsatellite markers were developed and characterized in a gynodioecious summer-deciduous shrub, Daphne jezoensis, to facilitate studies of the evolution of gynodioecy in the species. • METHODS AND RESULTS We used a next-generation sequencing approach with the Ion Personal Genome Machine (PGM) system to identify and develop microsatellite markers with perfect di- and trinucleotide repeats. These markers were tested with 47 samples from two natural populations. The mean observed and expected heterozygosities per population ranged from 0.40 to 0.46 and 0.60 to 0.66, respectively. • CONCLUSIONS The developed markers will be useful to study the mating system, gene flow, and population genetic structure of D. jezoensis.
منابع مشابه
Size-dependent sex allocation and reproductive investment in a gynodioecious shrub
In sexually dimorphic plants, resource allocation to reproduction often differs between sex morphs. In gynodioecious species, i.e. coexisting hermaphrodite and female plants within a population, females often produce more fruits than hermaphrodites. Since fruit production is costlier than flower production, hermaphrodites and females may regulate flower and fruit production differently in respo...
متن کاملEcological context of breeding system variation: sex, size and pollination in a (predominantly) gynodioecious shrub.
BACKGROUND AND AIMS Species that exhibit among-population variation in breeding system are particularly suitable to study the importance of the ecological context for the stability and evolution of gender polymorphism. Geographical variation in breeding system and sex ratio of Daphne laureola (Thymelaeaceae) was examined and their association with environmental conditions, plant and floral disp...
متن کاملSite-specific features affect pollination success of a gynodioecious understory shrub in a gender-specific mode1
Daphne laureola (Thymelaeaceae) is a gynodioecious, winter-flowering shrub inhabiting the understory of shady mountain forests. The small, inconspicuous yellowish-green flowers are pollinated by ectothermic insects whose activity is mainly confined to infrequent spells of sunny weather. We tested the hypotheses that (1) the solar irradiance environment of individual D. laureola plants and dista...
متن کاملHerbivores do not discriminate between leaves of female and hermaphrodite individuals of gynodioecious Daphne laureola (Thymelaeaceae)
The outcome of plant-animal interactions in dioecious plant species frequently depends on the gender of the plant individuals. It has even been proposed that these interactions could mediate the evolution of plant reproductive systems from hermaphroditism to dioecy. Gynodioecy is the most frequent intermediate stage in this evolutionary process, however, little is known about the relevance of g...
متن کاملNeither vegetative nor reproductive advantages account for high frequency of male-steriles in southern Spanish gynodioecious Daphne laureola (Thymelaeaceae).
In gynodioecious species, male-steriles (termed "females" hereafter) usually exhibit some reproductive advantage over hermaphrodites that allow them to compensate for the loss of male reproductive function. This compensation can result from higher fecundity, vegetative outperformance, and/or lower inbreeding depression. In this study we compared vegetative and reproductive parameters of female ...
متن کامل